Journal: Acta Neuropathologica Communications

Article Title: Intracellular dynamics of Ataxin-2 in the human brains with normal and frontotemporal lobar degeneration with TDP-43 inclusions

doi: 10.1186/s40478-020-01055-9

Figure Lengend Snippet: Colocalization coefficient values from all evaluated brain regions of the control cases

Article Snippet: For single- or double-immunofluorescence labeling of normal controls (cases 1, 2, and 3) and FTLD-TDP cases (cases 15, 16, and 17), brain sections were pretreated and incubated for 3 days with a cocktail containing one or two of the following primary antibodies: two anti-ATXN2 antibodies as mentioned above, anti-PolyA-binding protein 1 (PABP1) antibody (polyclonal, #4992, CST), anti-TDP-43 antibody (monoclonal, H00023435-M01, Avnova), several organelle markers including endoplasmic reticulum (ER) marker (anti-Calnexin, monoclonal, #2679, CST), ribosomal marker (anti-ribosomal protein S6 (RPS6), monoclonal, #2217, CST), lysosome marker (anti-Lysosome associated membrane protein 1 (LAMP1), polyclonal, ab24170, abcam), and Golgi apparatus marker (anti-Golgi Glycoprotein 1 (GLG1), polyclonal, ab103439, abcam).

Techniques: Control, Immunolabeling

Journal: Acta Neuropathologica Communications

Article Title: Intracellular dynamics of Ataxin-2 in the human brains with normal and frontotemporal lobar degeneration with TDP-43 inclusions

doi: 10.1186/s40478-020-01055-9

Figure Lengend Snippet: Colocalization images of ATXN2 in cytoplasmic organelle and related molecules in normal control brain. The confocal microscopic images of cytoplasmic ATXN2 and several organelle marker proteins, including endoplasmic reticulum marker Calnexin, ribosomal protein S6 (RPS6), Golgi Glycoprotein 1 (GLG1), Lysosome associated membrane protein 1 (LAMP1), ATXN2’s related protein poly-A binding protein 1 (PABP1) and TDP-43, are shown. a – f Representative fluorescent double-stained images from the upper layer of the cerebral temporal cortex, including merged images with DAPI staining and two-dimensional scatterplots. ATXN2 is partially localized within the endoplasmic reticulum ( a ) and is strongly associated with ribosomes ( b ) and PABP1 ( e ) in controls, while ATXN2 did not localize within the Golgi apparatus ( c ) and lysosomes ( d ). There was no colocalization between ATXN2 and TDP-43 ( f ). Pseudo coloring was used for each channel

Article Snippet: For single- or double-immunofluorescence labeling of normal controls (cases 1, 2, and 3) and FTLD-TDP cases (cases 15, 16, and 17), brain sections were pretreated and incubated for 3 days with a cocktail containing one or two of the following primary antibodies: two anti-ATXN2 antibodies as mentioned above, anti-PolyA-binding protein 1 (PABP1) antibody (polyclonal, #4992, CST), anti-TDP-43 antibody (monoclonal, H00023435-M01, Avnova), several organelle markers including endoplasmic reticulum (ER) marker (anti-Calnexin, monoclonal, #2679, CST), ribosomal marker (anti-ribosomal protein S6 (RPS6), monoclonal, #2217, CST), lysosome marker (anti-Lysosome associated membrane protein 1 (LAMP1), polyclonal, ab24170, abcam), and Golgi apparatus marker (anti-Golgi Glycoprotein 1 (GLG1), polyclonal, ab103439, abcam).

Techniques: Control, Marker, Membrane, Binding Assay, Staining

Journal: Acta Neuropathologica Communications

Article Title: Intracellular dynamics of Ataxin-2 in the human brains with normal and frontotemporal lobar degeneration with TDP-43 inclusions

doi: 10.1186/s40478-020-01055-9

Figure Lengend Snippet: Summary of colocalization coefficient values from all evaluated brain regions of the FTLD-TDP cases

Article Snippet: For single- or double-immunofluorescence labeling of normal controls (cases 1, 2, and 3) and FTLD-TDP cases (cases 15, 16, and 17), brain sections were pretreated and incubated for 3 days with a cocktail containing one or two of the following primary antibodies: two anti-ATXN2 antibodies as mentioned above, anti-PolyA-binding protein 1 (PABP1) antibody (polyclonal, #4992, CST), anti-TDP-43 antibody (monoclonal, H00023435-M01, Avnova), several organelle markers including endoplasmic reticulum (ER) marker (anti-Calnexin, monoclonal, #2679, CST), ribosomal marker (anti-ribosomal protein S6 (RPS6), monoclonal, #2217, CST), lysosome marker (anti-Lysosome associated membrane protein 1 (LAMP1), polyclonal, ab24170, abcam), and Golgi apparatus marker (anti-Golgi Glycoprotein 1 (GLG1), polyclonal, ab103439, abcam).

Techniques: Immunolabeling

Journal: Acta Neuropathologica Communications

Article Title: Intracellular dynamics of Ataxin-2 in the human brains with normal and frontotemporal lobar degeneration with TDP-43 inclusions

doi: 10.1186/s40478-020-01055-9

Figure Lengend Snippet: Semi-quantitative immunohistochemical analysis of human neuronal ATXN2. The brain sections of temporal neocortex from three control cases and three FTLD-TDP cases were immunostained with the antibodies for ATXN2, RPS6, and PABP1 by the same procedure. The signal intensities within each neuronal cytoplasm from the fluorescent images were semi-quantified and statistically compared. a Representative immunofluorescent images and automatically determined ROIs within each neuronal cytoplasm. b Integrated density and corrected total cell fluorescence (CTCF) values per unit area (μm 2 ) for ATXN2, RPS6, and PABP1 were calculated from fifty ROIs and plotted. Data are mean ± standard deviation, and statistical comparisons were made by unpaired Student T tests. The signal intensity of ATXN2 in the FTLD-TDP cases was significantly reduced compared with controls (* P ≤ 0.05), but its related molecules RPS6 and PABP1, showed no such significant reduction

Article Snippet: For single- or double-immunofluorescence labeling of normal controls (cases 1, 2, and 3) and FTLD-TDP cases (cases 15, 16, and 17), brain sections were pretreated and incubated for 3 days with a cocktail containing one or two of the following primary antibodies: two anti-ATXN2 antibodies as mentioned above, anti-PolyA-binding protein 1 (PABP1) antibody (polyclonal, #4992, CST), anti-TDP-43 antibody (monoclonal, H00023435-M01, Avnova), several organelle markers including endoplasmic reticulum (ER) marker (anti-Calnexin, monoclonal, #2679, CST), ribosomal marker (anti-ribosomal protein S6 (RPS6), monoclonal, #2217, CST), lysosome marker (anti-Lysosome associated membrane protein 1 (LAMP1), polyclonal, ab24170, abcam), and Golgi apparatus marker (anti-Golgi Glycoprotein 1 (GLG1), polyclonal, ab103439, abcam).

Techniques: Immunohistochemical staining, Control, Fluorescence, Standard Deviation

Journal: Acta Neuropathologica Communications

Article Title: Intracellular dynamics of Ataxin-2 in the human brains with normal and frontotemporal lobar degeneration with TDP-43 inclusions

doi: 10.1186/s40478-020-01055-9

Figure Lengend Snippet: Colocalization images of ATXN2 in cytoplasmic organelle and related molecules in normal control brain. The confocal microscopic images of cytoplasmic ATXN2 and several organelle marker proteins, including endoplasmic reticulum marker Calnexin, ribosomal protein S6 (RPS6), Golgi Glycoprotein 1 (GLG1), Lysosome associated membrane protein 1 (LAMP1), ATXN2’s related protein poly-A binding protein 1 (PABP1) and TDP-43, are shown. a – f Representative fluorescent double-stained images from the upper layer of the cerebral temporal cortex, including merged images with DAPI staining and two-dimensional scatterplots. ATXN2 is partially localized within the endoplasmic reticulum ( a ) and is strongly associated with ribosomes ( b ) and PABP1 ( e ) in controls, while ATXN2 did not localize within the Golgi apparatus ( c ) and lysosomes ( d ). There was no colocalization between ATXN2 and TDP-43 ( f ). Pseudo coloring was used for each channel

Article Snippet: For single- or double-immunofluorescence labeling of normal controls (cases 1, 2, and 3) and FTLD-TDP cases (cases 15, 16, and 17), brain sections were pretreated and incubated for 3 days with a cocktail containing one or two of the following primary antibodies: two anti-ATXN2 antibodies as mentioned above, anti-PolyA-binding protein 1 (PABP1) antibody (polyclonal, #4992, CST), anti-TDP-43 antibody (monoclonal, H00023435-M01, Avnova), several organelle markers including endoplasmic reticulum (ER) marker (anti-Calnexin, monoclonal, #2679, CST), ribosomal marker (anti-ribosomal protein S6 (RPS6), monoclonal, #2217, CST), lysosome marker (anti-Lysosome associated membrane protein 1 (LAMP1), polyclonal, ab24170, abcam), and Golgi apparatus marker (anti-Golgi Glycoprotein 1 (GLG1), polyclonal, ab103439, abcam).

Techniques: Control, Marker, Membrane, Binding Assay, Staining

Journal: Acta Neuropathologica Communications

Article Title: Intracellular dynamics of Ataxin-2 in the human brains with normal and frontotemporal lobar degeneration with TDP-43 inclusions

doi: 10.1186/s40478-020-01055-9

Figure Lengend Snippet: Primary antibodies used in this study

Article Snippet: For single- or double-immunofluorescence labeling of normal controls (cases 1, 2, and 3) and FTLD-TDP cases (cases 15, 16, and 17), brain sections were pretreated and incubated for 3 days with a cocktail containing one or two of the following primary antibodies: two anti-ATXN2 antibodies as mentioned above, anti-PolyA-binding protein 1 (PABP1) antibody (polyclonal, #4992, CST), anti-TDP-43 antibody (monoclonal, H00023435-M01, Avnova), several organelle markers including endoplasmic reticulum (ER) marker (anti-Calnexin, monoclonal, #2679, CST), ribosomal marker (anti-ribosomal protein S6 (RPS6), monoclonal, #2217, CST), lysosome marker (anti-Lysosome associated membrane protein 1 (LAMP1), polyclonal, ab24170, abcam), and Golgi apparatus marker (anti-Golgi Glycoprotein 1 (GLG1), polyclonal, ab103439, abcam).

Techniques:

Journal: European journal of medicinal chemistry

Article Title: Design, synthesis and neurite outgrowth activity of novel ganglioside GM1 derivatives by remodeling of the fatty acid moiety.

doi: 10.1016/j.ejmech.2022.114636

Figure Lengend Snippet: Fig. 6. GM1 (d18:1) and GM1 (d18:1/C16:1) promote cholesterol biosynthesis through ERBB4 and the mTOR pathway. (A–B) Immunoblotting of extracts from Neuro2a cells treated as indicated, with the indicated antibodies (A), with band quantification (B). (C–D) Immunoblotting with the indicated antibodies (mTOR, ERBB4 and TrkA). (C) with band quantification (D) of extracts from Neuro2a cells treated as indicated. (E–F) Neuro2a cells were treated with GM1 (d18:1) and GM1 (d18:1/C16:1). Lipid raft and non-raft fractions were isolated and analysed by immunoblotting. Lipid raft was revealed using anti-flotillin-1 antibody, and non-raft part was indicated using anti-β-ACTIN antibody.

Article Snippet: Five antibodies for the selected proteins were used: GAP43 Rabbit mAb (Cell Signal Technology, Cat No. 8945, 1:500 in PBS), SREBP2 Rabbit PolyAb (Proteintech Group, Cat No. 14508-1- AP, 1:500 in PBS), LSS Rabbit PolyAb (Proteintech Group, Cat No. 13715-1- AP, 1:1500 in PBS), HMGCR Rabbit PolyAb (abcam, Cat No. ab174830, 1:1000 in PBS), TrkA Rabbit PolyAb (abcam, Cat No. ab76291, 1:1000 in PBS), ERBB4 Rabbit mAb (Cell Signal Technology, Cat No. 4795, 1:1000 in PBS), p-ERBB4 Rabbit mAb (abcam, Cat No. ab76132, 1:2000 in PBS), Flotillin-1 Rabbit mAb (Cell Signal Technology, Cat No. 18634, 1:1000 in PBS), p-PI3K Rabbit PolyAb (abcam, Cat No. ab197071 in PBS), and mTOR Rabbit PolyAb (Proteintech, Cat No. 20657-1- AP, 1:1000 in PBS). β-ACTIN Rabbit mAb (Cell Signal Technology, Cat No. 4970, 1:1000 in PBS) was used to detect the internal reference β-ACTIN.

Techniques: Western Blot, Isolation

Journal: European journal of medicinal chemistry

Article Title: Design, synthesis and neurite outgrowth activity of novel ganglioside GM1 derivatives by remodeling of the fatty acid moiety.

doi: 10.1016/j.ejmech.2022.114636

Figure Lengend Snippet: Fig. 7. Model for how GM1 (d18:1) and GM1 (d18:1/C16:1) induce transcription of the SREBP2 gene by activating ERBB4-mediated PI3K-mTOR signaling. Exogenous GM1 and its derivatives activate the ERBB4 or TrkA receptors on the cell membrane surface, which in turn activates the downstream PI3K-mTORC1 signaling pathway, which in turn activates the SREBP2 gene, thereby regulating the expression of cholesterol biosynthesis related genes such as HMGCR, MVK, and LSS. Enhance the synthesis of cholesterol in the cell and increase the content of cholesterol in the cell. This figure was created with Biorender.com.

Article Snippet: Five antibodies for the selected proteins were used: GAP43 Rabbit mAb (Cell Signal Technology, Cat No. 8945, 1:500 in PBS), SREBP2 Rabbit PolyAb (Proteintech Group, Cat No. 14508-1- AP, 1:500 in PBS), LSS Rabbit PolyAb (Proteintech Group, Cat No. 13715-1- AP, 1:1500 in PBS), HMGCR Rabbit PolyAb (abcam, Cat No. ab174830, 1:1000 in PBS), TrkA Rabbit PolyAb (abcam, Cat No. ab76291, 1:1000 in PBS), ERBB4 Rabbit mAb (Cell Signal Technology, Cat No. 4795, 1:1000 in PBS), p-ERBB4 Rabbit mAb (abcam, Cat No. ab76132, 1:2000 in PBS), Flotillin-1 Rabbit mAb (Cell Signal Technology, Cat No. 18634, 1:1000 in PBS), p-PI3K Rabbit PolyAb (abcam, Cat No. ab197071 in PBS), and mTOR Rabbit PolyAb (Proteintech, Cat No. 20657-1- AP, 1:1000 in PBS). β-ACTIN Rabbit mAb (Cell Signal Technology, Cat No. 4970, 1:1000 in PBS) was used to detect the internal reference β-ACTIN.

Techniques: Membrane, Expressing

Journal: Materials Today Bio

Article Title: Oral microbiota-responsive ZIF nanoplatform via double-layer glycans modified combined with PD-1 inhibitor for treatment of microsatellite-stable colorectal cancer

doi: 10.1016/j.mtbio.2025.102565

Figure Lengend Snippet: In vitro therapeutic efficacy of (In/Cos)@Ua-Rg3-ZIF. Effects of Ua-Rg3-ZIF on (A) DC2.4 and (B) CT-26 cell viability (n = 3). Effects of (In/Cos)@Ua-Rg3-ZIF on (C) DC2.4 and (D) CT-26 viability (n = 3).(E) CRT and (F) HMGB1 expression profiles in CT-26 cells visualized by CLSM (Scale bar = 100 nm) (G) Flow cytometry analysis of MHC-II, CD86, and CD40 expression levels on DC2.4 cells co-cultured with CT-26 (n = 3).(H) Flow cytometry analysis of MHC-II, CD86, and CD40 expression levels on BMDCs co-cultured with CT-26 (n = 3). Data are expressed as the mean ± SD (∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; one-way ANOVA).

Article Snippet: Antibodies for FACS analysis, including KO525 Fixable Viability Dye, Purified anti-mouse CD16/32 Antibody, PB450 anti-mouse CD45 Antibody, FITC anti-mouse CD3 Antibody, BV510 anti-mouse CD4 Antibody, APC anti-mouse CD8a Antibody, PE anti-mouse Granzyme B Antibody, PC7 anti-mouse IFN-γ Antibody, APC anti-mouse CD11c Antibody, APC-A700 anti-mouse I-A/I-E Antibody, and PE anti-mouse CD86 Antibody were sourced from Biolegend Inc. HMGB1 Rabbit PolyAb and Calreticulin Rabbit PolyAb were sourced from Proteintech Group, Inc.

Techniques: In Vitro, Drug discovery, Expressing, Flow Cytometry, Cell Culture

Journal: Materials Today Bio

Article Title: Oral microbiota-responsive ZIF nanoplatform via double-layer glycans modified combined with PD-1 inhibitor for treatment of microsatellite-stable colorectal cancer

doi: 10.1016/j.mtbio.2025.102565

Figure Lengend Snippet: Mechanism of the combined antitumor effect of (In/Cos)@Ua-Rg3-ZIF with PD-1 blockade in vivo . (A) Expression levels of CRT and (B) HMGB1 in tumor tissues of Balb/c mice after various treatments (Scale bar = 1000 μm). (C) Proportions of CD86 + and (D) MHC-II + cells among CD11c + DCs quantified by flow cytometry (n = 3). (E) Proportion of CD3 + cells within CD45 + TILs (n = 3). (F) Proportion of CD4 + cells within CD3 + TILs (n = 3). (G) Proportions of Granzyme B + and (H) IFN-γ + cells within CD8 + TILs analyzed by flow cytometry (n = 3). Data are expressed as the mean ± SD (∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; one-way ANOVA).

Article Snippet: Antibodies for FACS analysis, including KO525 Fixable Viability Dye, Purified anti-mouse CD16/32 Antibody, PB450 anti-mouse CD45 Antibody, FITC anti-mouse CD3 Antibody, BV510 anti-mouse CD4 Antibody, APC anti-mouse CD8a Antibody, PE anti-mouse Granzyme B Antibody, PC7 anti-mouse IFN-γ Antibody, APC anti-mouse CD11c Antibody, APC-A700 anti-mouse I-A/I-E Antibody, and PE anti-mouse CD86 Antibody were sourced from Biolegend Inc. HMGB1 Rabbit PolyAb and Calreticulin Rabbit PolyAb were sourced from Proteintech Group, Inc.

Techniques: In Vivo, Expressing, Flow Cytometry

Journal: European journal of medicinal chemistry

Article Title: Design, synthesis and neurite outgrowth activity of novel ganglioside GM1 derivatives by remodeling of the fatty acid moiety.

doi: 10.1016/j.ejmech.2022.114636

Figure Lengend Snippet: Fig. 4. GM1 (d18:1) and GM1 (d18:1/C16:1) induce the expression of sterol-related genes. (A) STRING analysis of the top-20-ranking upregulated differentially expressed genes from an RNA-seq analysis of Neuro2a cells treated with GM1 (d18:1/C16:1), GM1(d18), or vehicle control. (B) STRING analysis of the top-20-ranking downregulated differentially expressed genes. (C) STRING analysis of SREBP2. (D) qPCR analysis of the indicated genes in Neuro2a cells treated GM1 (d18:1) and GM1 (d18:1/C16:1). (E) PRM results for the indicated proteins in Neuro2a cells treated with the indicated GM1 species (GM1 (d18:1) and GM1 (d18:1/C16:1). (F–G) Total cholesterol and cholesteryl ester levels of Neuro2a Cells. Statistical analyses were performed using Mann–Whitney U tests. Statistical analyses were performed using the Mann–Whitney U tests. All data are shown as the mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: Five antibodies for the selected proteins were used: GAP43 Rabbit mAb (Cell Signal Technology, Cat No. 8945, 1:500 in PBS), SREBP2 Rabbit PolyAb (Proteintech Group, Cat No. 14508-1- AP, 1:500 in PBS), LSS Rabbit PolyAb (Proteintech Group, Cat No. 13715-1- AP, 1:1500 in PBS), HMGCR Rabbit PolyAb (abcam, Cat No. ab174830, 1:1000 in PBS), TrkA Rabbit PolyAb (abcam, Cat No. ab76291, 1:1000 in PBS), ERBB4 Rabbit mAb (Cell Signal Technology, Cat No. 4795, 1:1000 in PBS), p-ERBB4 Rabbit mAb (abcam, Cat No. ab76132, 1:2000 in PBS), Flotillin-1 Rabbit mAb (Cell Signal Technology, Cat No. 18634, 1:1000 in PBS), p-PI3K Rabbit PolyAb (abcam, Cat No. ab197071 in PBS), and mTOR Rabbit PolyAb (Proteintech, Cat No. 20657-1- AP, 1:1000 in PBS). β-ACTIN Rabbit mAb (Cell Signal Technology, Cat No. 4970, 1:1000 in PBS) was used to detect the internal reference β-ACTIN.

Techniques: Expressing, RNA Sequencing, Control, MANN-WHITNEY

Journal: European journal of medicinal chemistry

Article Title: Design, synthesis and neurite outgrowth activity of novel ganglioside GM1 derivatives by remodeling of the fatty acid moiety.

doi: 10.1016/j.ejmech.2022.114636

Figure Lengend Snippet: Fig. 5. GM1 (d18:1) and GM1 (d18:1/C16:1) promote cholesterol biosynthesis through SREBP2. (A) Representative images of Neuro2A cells treated with each species for 24 h. (B) The percentage of cells with neurites upon incubation with the indicated GM1 species (two components of GM1, GM1 (C16:1) and GM1 (C18:1). (C) Quantification of neurite number per cell from B. (D) Length of the longest neurite per Cell from B. (E) PRM results for the indicated proteins in SREBP2-shRNAi cells treated with the indicated GM1 species (GM1 (d18:1) and GM1 (d18:1/C16:1). (F–G) Immunoblotting with the indicated antibodies and band quantification of extracts from SREBP2-shRNAi cells. Statistical analyses were performed using Man n–Whitney U tests. All data are shown as the mean ± SD from three independent experiments. *p < 0.05, **p < 0.01,***p < 0.001.

Article Snippet: Five antibodies for the selected proteins were used: GAP43 Rabbit mAb (Cell Signal Technology, Cat No. 8945, 1:500 in PBS), SREBP2 Rabbit PolyAb (Proteintech Group, Cat No. 14508-1- AP, 1:500 in PBS), LSS Rabbit PolyAb (Proteintech Group, Cat No. 13715-1- AP, 1:1500 in PBS), HMGCR Rabbit PolyAb (abcam, Cat No. ab174830, 1:1000 in PBS), TrkA Rabbit PolyAb (abcam, Cat No. ab76291, 1:1000 in PBS), ERBB4 Rabbit mAb (Cell Signal Technology, Cat No. 4795, 1:1000 in PBS), p-ERBB4 Rabbit mAb (abcam, Cat No. ab76132, 1:2000 in PBS), Flotillin-1 Rabbit mAb (Cell Signal Technology, Cat No. 18634, 1:1000 in PBS), p-PI3K Rabbit PolyAb (abcam, Cat No. ab197071 in PBS), and mTOR Rabbit PolyAb (Proteintech, Cat No. 20657-1- AP, 1:1000 in PBS). β-ACTIN Rabbit mAb (Cell Signal Technology, Cat No. 4970, 1:1000 in PBS) was used to detect the internal reference β-ACTIN.

Techniques: Incubation, Western Blot

Journal: European journal of medicinal chemistry

Article Title: Design, synthesis and neurite outgrowth activity of novel ganglioside GM1 derivatives by remodeling of the fatty acid moiety.

doi: 10.1016/j.ejmech.2022.114636

Figure Lengend Snippet: Fig. 7. Model for how GM1 (d18:1) and GM1 (d18:1/C16:1) induce transcription of the SREBP2 gene by activating ERBB4-mediated PI3K-mTOR signaling. Exogenous GM1 and its derivatives activate the ERBB4 or TrkA receptors on the cell membrane surface, which in turn activates the downstream PI3K-mTORC1 signaling pathway, which in turn activates the SREBP2 gene, thereby regulating the expression of cholesterol biosynthesis related genes such as HMGCR, MVK, and LSS. Enhance the synthesis of cholesterol in the cell and increase the content of cholesterol in the cell. This figure was created with Biorender.com.

Article Snippet: Five antibodies for the selected proteins were used: GAP43 Rabbit mAb (Cell Signal Technology, Cat No. 8945, 1:500 in PBS), SREBP2 Rabbit PolyAb (Proteintech Group, Cat No. 14508-1- AP, 1:500 in PBS), LSS Rabbit PolyAb (Proteintech Group, Cat No. 13715-1- AP, 1:1500 in PBS), HMGCR Rabbit PolyAb (abcam, Cat No. ab174830, 1:1000 in PBS), TrkA Rabbit PolyAb (abcam, Cat No. ab76291, 1:1000 in PBS), ERBB4 Rabbit mAb (Cell Signal Technology, Cat No. 4795, 1:1000 in PBS), p-ERBB4 Rabbit mAb (abcam, Cat No. ab76132, 1:2000 in PBS), Flotillin-1 Rabbit mAb (Cell Signal Technology, Cat No. 18634, 1:1000 in PBS), p-PI3K Rabbit PolyAb (abcam, Cat No. ab197071 in PBS), and mTOR Rabbit PolyAb (Proteintech, Cat No. 20657-1- AP, 1:1000 in PBS). β-ACTIN Rabbit mAb (Cell Signal Technology, Cat No. 4970, 1:1000 in PBS) was used to detect the internal reference β-ACTIN.

Techniques: Membrane, Expressing